Tris Hydrochloride

CAS No.: 1185-53-1

Chemical formula: C4H12ClNO3

Molecular weight: 157.6

EINECS No.: 214-684-5

Melting point: 150-152 °C

Boiling point: 225℃[at 101 325 Pa]

Density: 1.05 g/mL at 20 °C

Vapor Pressure: 0Pa at 20℃

Storage conditions: room temp

Solubility: H2O: 4 M at 20 °C, clear, colorless

Form: crystalline

Color: clear colorless (40 % (w/w) in H2O) solution

PH value: 2.5-4.0 (25℃, 4M in H2O)

Stability: Stable. Incompatible with strong oxidizing agents, bases, moisture.

Toxicity: low toxicity, can cause cancer, do not directly contact the skin

 

Uses: Tris hydrochloride is referred to as Tris-HCl. Tris hydrochloride buffer (Tris-HCl buffer) is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. Used in the preparation of buffer solutions in biochemical and molecular biology experiments; pharmaceutical intermediates; used in the preparation of surfactants and vulcanization accelerators; prepared water-soluble polymers containing Tris structural units as paint dispersants; indoor Preparation of formaldehyde adsorption materials, etc.

Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important uses. Tris was used for protein crystal growth under different pH conditions. The low ionic strength of Tris buffer can be used for the formation of intermediate fibers of C. elegans nuclear lamin. Tris is also one of the main components of the protein electrophoresis buffer, which forms a buffer system with glycine in the electrophoresis buffer to stabilize the pH during electrophoresis. In addition, Tris is also an intermediate for the preparation of surfactants, vulcanization accelerators and some drugs. Tris was also used as a titration standard. When used as a protein buffer, if mass spectrometry is required for follow-up work, Tris is not suitable, and it is better to replace it with a buffer that can be tolerated by other mass spectrometers.

 

Product advantages: purity ≥ 99%, sufficient inventory, good water solubility, and stable process.

 

 

PIC: Pepper Wang
Email: km12@kmchem.com
WhatsApp/Mobile: +86 15563229915

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